期刊论文

中文

基础医学与临床

1001-6325

2002

22

1

88-91

10.3969/j.issn.1001-6325.2002.01.018

湖南省教委资助课题 （99C1 93）；

本校为第一机构

为阐明一氧化氮途径对精子发生的影响,探讨一氧化氮对精子发生的调节作用,将雄性SD大鼠分为4组,分别于腹腔内注射左旋精氨酸(L-arginine,L-ARG)、N-硝基左旋精氨酸甲酯(N-nitro-L-arginine-mythel-ester,L-NAME)、L-ARG+L-NAME与生理盐水,每天一次,共12d.于最后一次注射后2h采血并处死动物.放免法测定血清内睾酮含量;GREISS法测定血清NO-x(硝酸盐/亚硝酸盐)含量;常规组织学切片观察生精上皮形态,并对Ⅶ-Ⅷ期生精上皮横断面的各级生精细胞进行定量分析;扫描电镜观察生精小管内精子密度.结果表明:L-ARG组血清内NO-x含量高于对照组(P＜0.01),睾酮含量低于对照组(P＜0.01),精子生成减少(P＜...

s: The objective of the present study was to evaluate prospectively the role of nitric oxide (NO) in modulating spermatogenesis. Fourty male rats(60～70days) were individed into four groups. Ten male rats were injected intraperitoneally one of following agents(once a day,for 12 days): (1) L arginine(L ARG); (2) N nitro L arginine mythel ester(L NAME); (3)L ARG+L NAME;(4)Normal saline. The blood samples were collected at two hours after postinjection and the rats were crucified. The concentration of serum testosterone was measured by radioimmuno...