Efficient mutagenesis method for producing the templates of single nucleotide polymorphisms

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Zhang, J;Li, K;Deng, ZM;Liao, DF;Fang, WY;...

通讯作者：

Kai Li

作者机构：

Nanhua Univ, Inst Pharmacol & Pharm, Inst Biotechnol, Hengyang 421001, Peoples R China.

Charles R Drew Univ Med & Sci, Vasc Biol Lab, Los Angeles, CA 90059 USA.

Jinan Univ, Inst Life Sci & Biotechnol, Guangzhou, Peoples R China.

Genomapping Inc, Tianjin, Peoples R China.

[Zhang, J; Liao, DF; Fang, WY] Institute of Biotechnology, Institute of Pharmacology and Pharmacy, Nanhua University, Hengyang, China

通讯机构：

[Kai Li] I

Institute of Biotechnology, Institute of Pharmacology and Pharmacy, Nanhua University, Hengyang, China<&wdkj&>Vascular Biology Laboratory, Charles R. Drew University of Medicine and Science, Los Angeles

语种：

英文

关键词：

mutagenesis;single nucleotide polymorphisms

期刊：

Molecular Biotechnology

ISSN：

1073-6085

年：

2003

卷：

期：

页码：

105-110

DOI：

10.1385/MB:24:2:105

机构署名：

本校为第一机构

院系归属：

药学与生物科学学院

摘要：

DNA templates harboring specific single nucleotide polymorphism (SNP) sites are largely needed as positive controls in practical SNP analysis and in determination of the reliability of newly developed methods in high-throughput screening assays. Here we report a one-step method to produce SNP templates by amplifying a wild-type sequence with primers having single nucleotide mismatches at or near their 3' ends. A short amplicon harboring an EcoRI site was used to evaluate the feasibility of our strategy. Perfectly matched primers and primers with a single base mismatch occurring from the first ...

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Efficient mutagenesis method for producing the templates of single nucleotide polymorphisms

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