Construct a sensitive and rapid detection fluorescence biosensor with the assistance of exonuclease III to achieve efficient detection of Hg(2+). Nucleic acid aptamer H0 specifically recognizes Hg(2+), and under the action of exonuclease III, H0 bound to Hg(2+) is hydrolyzed, which in turn fails to trigger the catalytic hairpin self-assembly amplification reaction, resulting in a decrease in the number of double-stranded DNA bound to the fluorescent dye SYBR Green I in the solution, and a decrease in the fluorescence intensity. The results showed that the concentration of Hg(2+) showed a good ...
