期刊论文

Effects of Rotenone on Cognitive Function as well as Hippocampal Warburg Effect and Microglia Polarization in Rats

中文

中山大学学报(医学科学版)

1672-3554

2021

42

06

801-807

10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2021.0601

国家自然科学基金（81771178）； 湖南省自然科学基金（2020JJ4553）； 湖南省临床医疗技术创新引导项目（2020SK51908）；

本校为第一机构

【目的】探究鱼藤酮(ROT)对大鼠认知功能及其海马Warburg效应和小胶质细胞极化的影响。 【方法】雄性SD大鼠随机分为对照组和ROT处理组。ROT处理组大鼠颈背部皮下注射ROT(2 mg/kg),对照组注射等量的溶剂。Y迷宫实验和新物体识别实验评估大鼠的认知功能,Western blot检测海马Warburg效应相关蛋白己糖激酶2(HK2)、丙酮酸激酶同工酶2(PKM2)、丙酮酸脱氢酶激酶1(PDK-1)、丙酮酸脱氢酶(PDH)、乳酸脱氢酶A(LDHA)和小胶质细胞极化标志性蛋白诱导型一氧化氮合成酶(iNOS)、精氨酸酶1(Arg-1)、几丁质酶3样分子3(Ym-1)的表达水平,乳酸试剂盒检测海马组织乳酸含量,Griess法测定海马组织一氧化氮(NO)含量,酶联...

【Objective】To investigate the effects of rotenone(ROT)on cognitive function of rats,as well as on Warburg effect and microglia polarization in the hippocampus of rats.【Methods】Male Sprague Dawley(SD)rats were randomly divided into ROT-treated group and control group which were subcutaneously injected with ROT(2 mg/kg)and same volume of saline respectively. The cognitive function of rats was evaluated by Y-maze test and novel object recognition (NOR)test. Western blot(WB)was conducted to detect the expression levels of Warburg effect related...