期刊论文

Analysis on the polymorphisms of human B cell epitopes of 5 specificity antigens in Mycobacterium tuberculosis in China

中文

中华结核和呼吸杂志

1001-0939

2016

39

5

372-377

10.3760/cma.j.issn.1001-0939.2016.05.008

国家科技重大专项课题（2013ZX10003006-002-001） 国家自然科学基金（81401647）

本校为第一机构

目的 研究我国结核分枝杆菌复合群(MTBC)抗原mpt83、mpt70、lpqH、pstS3、groES及其人B细胞表位的多态性.方法 从中国疾病预防控制中心传染病所结核室菌株库随机选取我国的179株MTBC临床分离株,培养后提取DNA,基因扩增并测序,根据H37Rv标准序列和免疫表位数据库(IEDB)的抗原表位信息,比较、分析各基因B细胞表位序列的变异情况,最后用MEGA6软件计算各基因的非同义突变率(dN)、同义突变率(dS)及其比值.结果 在179株MTBC菌株中,mpt83基因有7个单核苷酸多态性(SNP)位点,该基因及其表位区、非表位区的dN/dS分别为0.88、0.34和0.14;mpt70基因有1个SNP位点,为非同义突变;lpqH基因及其B细胞表位区dN/dS均...

OBJECTIVE: To study the diversities of human B cell epitopes of five proteins (Mpt83, Mpt70, LpqH, PstS3, GroES) in Mycobacterium tuberculosis isolates from China. METHODS: We selected 179 strains isolated from patients with tuberculosis in different regions of China, and cultured these strains by L-J medium. The gene sequences of these 5 proteins were amplified and sequenced by PCR. The variations (single nucleotide polymorphisms, SNP) of the DNA sequences were compared and analyzed according to the immune epitope database (IEDB). The synonymous mutation rate (dS), non-synonymous mutation rat...