期刊论文

Su, Q.

中文

中国肿瘤临床

1000-8179

2010

37

3

121-125

10.3969/j.issn.1000-8179.2010.03.001

国家自然科学基金（编号:30600285）； 湖南省科研计划重大专项基金（编号:04SK1004）； 湖南省自然科学重点基金资助（编号:07JJ3033）~~；

本校为第一且通讯机构

目的:以细胞周期作为抗癌药物新靶点的研究,可能是很有前途的.笔者的前期工作发现,二烯丙基二硫化物(diallyl disulfide,DADS)可抑制人胃癌BGC823细胞增殖,其增殖抑制与细胞周期G_2/M期阻滞有关;DADS可能是通过抑制细胞分裂周期蛋白25C(Celldivision cycle protein25C,Cdc25C)、cyclinB1表达使部分BGC823细胞停滞在G_2/M期,但G_2/M期阻滞的机制还未完全阐明.本研究进一步探讨DADS诱导人胃癌BGC823细胞周期G_2/M期阻滞的可能机制.方法:RT-PCR检测Chk1和Chk2在mRNA水平的改变;Western blot检测DADS处理BGC823细胞前后细胞周期相关蛋白ATM-RAD3相关基因(ATM-RAD3-related gene,ATR)、细胞周期检查点...

Objective: Cell cycle has recently become more appealing as a new target of anti-carcinogenic agent. Diallyl disulfide (DADS) inhibits growth and induces cell cycle G2/M arrest in human gastric cancer BGC823 cells. Cell division cycle protein 25C (Cdc25C) and CyclinB1 expression are involved in G2/M arrest. However, mechanisms of G2/M arrest are not yet fully understood. The aim of this study was to elucidate the mechanism of cell cycle G2/M arrest in human gastric cancer BGC823 cells induced by DADS. Methods: The expression of Chk1 and Chk2 mRNA associated with cell cycle arrest of BGC823 cel...