期刊论文

中文

中国肿瘤临床

1000-8179

2000

27

2

104-107

10.3969/j.issn.1000-8179.2000.02.007

本校为第一机构

目的 :探讨c -Ha -ras基因突变及 p21蛋白表达与妊娠滋养细胞疾病 （GTD）发生发展的关系 ,寻找具有特异性的可预测PTD的诊断标记。方法 :采用PCR -SSCP法及SP免疫组化法 ,对90例GTD组织中c -Ha -ras基因第1外显子点突变及其产物 p21表达进行检测 ,以正常足月新鲜胎盘30例为对照。结果 :所有正常胎盘组织ras突变及p21表达均为阴性。ras基因突变在恶性GTD中为36 7 % ,CM将来发展成为PTD的为43 3 % ,均高于CM无PTD发展的16 7 %。恶性GTD,Ⅰ、Ⅱ、Ⅲ级组织分化者点突变率分别为15 4 %、40 %、71 4 % ,各级间差异有显著性 （P

Objective: To study the relationship between the mutation of C-Ha-ras gene and gestational trophoblastic disease (GTD) and to identify diagnostic marker for predicting GTD Methods: The mutation in exon 1 of C-Ha-ras gene and ras p21 expression in 90 cases of GTD and 30 normal placenta were determined by polymerase chain reaction-single strand conformation polymorphism analysis (PCR-SSCP) and streptavidin peroxidase conjugated method (SP) Results: All of the normal placenta were negative on the mutation in exon 1 of C-Ha-ras gene and p21 express...