This project was supported of Hunan Province （No.06jj5046, by the Natural Science Fund No.05jj30039） Acknowledgment We are indebted to Dr. Claus W. Heizmann for providing the primary rabbit anti-human S100A13 protein poiyclonal antibody.
机构署名：
本校为第一机构
院系归属：
医学院
摘要：
Objective To investigate the effect of exogenous S100A13 gene overexpression on the proliferation of human thyroid cancer cell line TT.Methods The recombinant ORF of S100A13 tagged with six histidines at the 5' end was subcloned into the pcDNA3.2NV5/GW/D-TOPO vector and sequenced.The eukaryotic expression plasmid pcDNA3.2/V5 /GW/D-S100A13 and empty vector pcDNA3.2/V5/GW/D were transfected into TT cells.The positive clones were selected by G418.The expressions of S100A13 mRNA and protein were detected by real-time reverse transcription-polymerase chain reaction (RT-PCR)and Western blot.The effe...
