期刊论文

中文

中国现代医学杂志

1005-8982

2009

19

5

662-664,667

10.3969/j.issn.1005-8982.2009.05.006

(06fi3119):湖南省科技厅科技计划项目

本校为第一机构

目的 探讨芹菜素(apigenin,API)抑制人肝癌Bel-7404细胞活性作用是否涉及Akt信号传导.方法 体外培养Bel-7404细胞,MTT比色法测定细胞活性;PI染色流式细胞术分析细胞周期;Western Blot分析细胞p-Akt、cyclin D1蛋白表达.结果 API抑制Bel-7404细胞活性,呈浓度依赖性,其IC50值为59.4μmol/L.API阻滞Bel-7404细胞周期于G_1期.API与PI3K特异性阻断剂LY294002均能有效下调Bel-7404细胞磷酸化Akt蛋白和cyclin D1蛋白表达.结论 API抑制Bel-7404细胞活性作用与其抑制Akt信号传导有关.

[Objective] To investigate effects of apigenin on the growth of human hepatoeellular carcinoma Bel- 7404 cell line and its mechanism whether or not be involved in Akt signal pathway. [Methods] Bel-7404 cell line was cultured in vitro. Cell viability was examined by MTr assay. Flow eytometry using PI staining was used to anal- yse cell cycle change. Western blot was used to analyze protein expression of p-Akt and cyclin D1 in Bel-7404 cells. [Results] Apigenin signitieanfly suppressed the cell viability of Bel-7404 cell line in a dose-dependant ...