期刊论文

中文

中国现代医学杂志

1005-8982

2009

19

21

3208-3211

10.3969/j.issn.1005-8982.2009.21.003

本校为第一机构

目的 探讨诱导性一氧化氮合酶(inducible nitric oxide synthase,iNOS)在2型糖尿病大鼠海马中的表达情况以及米诺环素对iNOS表达的影响.方法 高糖高脂饮食加小剂量链脲佐菌素建立2型糖尿病模型.将SD大鼠随机分为正常对照组(N组)、糖尿病模型组(D组)、米诺环素组(M组).造模成功1周后,M组每天腹腔注射米诺环素45mg/kg,D组和N组腹腔注射等量的生理盐水,连续给药2周,然后取大鼠海马组织,免疫组化方法测各组大鼠海马中iNOS表达情况.结果 N组、D组、M组海马iNOS阳性神经元的平均光密度值(MOD值)分别为(0.101±0.012)、(0.196±0.020)和(0:128±0.010),D组和M组iNOS表达较N组高,差异有显著性(P

[Objective] To explore the expression of inducible nitric oxide synthase （iNOS） in the hippocampus in a type 2 diabetes model of rats and the changes after the treatment with Minocycline. [ Methods ] A model of type 2 diabetes mellitus was induced by a high-glucose and high-fat diet and low doses of STZ. Rats were then randomly divided into 3 groups： control group, DM group and Minocycline group. One week after the model was set up, Minocycline at a dose of 45 mg/kg was administered intraperitoneally to the Minocycline group once a day for 2...