期刊论文

中文

中国现代医学杂志

1005-8982

2009

19

12

1793-1796

10.3969/j.issn.1005-8982.2009.12.009

湖南省科技厅科技计划项目

本校为第一机构

目的 观察芹菜素(apigenin,API)诱导人肝癌HepG2细胞凋亡的作用,研究其作用机制是否涉及PTEN蛋白表达的调控.方法 体外培养HepG2细胞,PI染色流式细胞术分析凋亡率;琼脂糖凝胶电泳观察DNA梯形条带;Western Blot分析细胞PTEN、p-Akt和p-Bad蛋白表达.结果 API诱导HepG2细胞的凋亡,呈浓度依赖性.40μmol/L的API处理HepG2细胞48 h,琼脂糖凝胶电泳呈现典型DNA梯形条带.40μmol/L的API处理HepG2细胞12 h和24 h,PTEN蛋白表达分别上调达132.6%和158.9%;同时磷酸化Akt蛋白水平下降至86.3%和75.6%.磷酸化Bad蛋白水平降低到73.4%和69.3%.结论 API诱导HepG2细胞凋亡作用与上调PTEN蛋白表达、降低磷酸化Akt蛋白...

[Objective] To investigate effects of Apigenin on apoptosis of human hepatocellular carcinoma HepG2 ceU line and its mechanism whether or not be involved in regulation of PTEN protein expression. [Methods] HepG2 cells were cultured in vitro. Flow cytometry using propidium iodide （PI） staining was used to determine cell apoptotic rate. DNA ladder bands were observed by DNA agarose gel electrophoresis. Western blot was used to analyze expression of PTEN, p-Akt and p-Bad proteins in HepG2 cells. [Results] FCM with PI staining demonstrated that A...