期刊论文

中文

中国现代医学杂志

1005-8982

2009

19

11

1640-1642

10.3969/j.issn.1005-8982.2009.11.011

本校为其他机构

目的 探讨苯对胚胎肢芽、中脑细胞分化和增殖的影响及对肢芽器官发育的影响.方法 分离12d龄小鼠胚胎肢芽和中脑细胞进行微团培养,观察肢芽和中脑细胞的分化与增殖;结果 随着苯染毒浓度的增加,胚胎肢芽细胞分化为软骨细胞的集落数逐渐减少,中脑细胞形成神经集落数减少、体积小,集落细胞间突起数量减少,肢芽细胞和中脑细胞增殖率下降,均具有较明显的剂量-效应关系;苯对肢芽细胞的半数分化抑制浓度(dID_(50))和半数增殖抑制浓度(pID_(50))分别为89.93μmol/L和396.77μmol/L,对中脑细胞的dID_(50)和pID_(50)分别为116.29μmol/L和831.46μmol/L,对细胞分化的抑制作用大于对增殖的抑制作用.结论 苯对...

[Objective] To explore the effect of Benzene on differentiation and proliferation of limb bud and midbrain cells. [Methods] lamb bud and midbrain cells were separated from mouse embryo at 12--day gestational age. Mieromass culture was used to investigate the effects of Benzene on differentiation and proliferation of embryo limb bud cells and midbrain cells. [Results] With the increasing of Benzene concentration, the proliferation and differentiation of embryo limb bud and midbrain cells were poorer and poorer in vitro, and there was the dose-ef...