期刊论文

中文

中国现代医学杂志

1005-8982

2008

18

1

65-68

10.3969/j.issn.1005-8982.2008.01.018

湖南省卫生厅科研基金资助（编号C2006-021）

本校为第一机构

目的探讨溶酶体组织蛋白酶B（cathepsin b，CB）在肺纤维化发病中的作用。方法应用博莱霉素（5mg／kg）气管内注入建立实验性大鼠肺纤维化模型，采用HE染色、Masson三联染色、免疫组织化学染色、RT-PCR等方法，观察实验性大鼠肺纤维化的发病过程及其肺组织中Cathepsin B蛋白及mRNA表达水平的动态变化。结果实验性大鼠肺纤维化发病过程中，呈现典型的肺泡炎（7d）、纤维组织增生（14d）及稳定的肺纤维化（28—35d）等表现；Cathepsin B在正常肺组织表达较弱，在肺纤维化组7d时表达增强，14d进一步增强，28d明显增强，35d时较28d开始减弱，但仍强于正常组；Cathepsin B mRNA在正常肺组织中表达较弱...

[Objective] To investigate the role of CB protein in the pathogenesis of experimental pulmonary fibrosis. [Methods] 48 male SD rats were randomly divided into two groups, the pulmonary fibrosis group and the control group. Rat pulmonary fibrosis was reproduced by an intratracheal injection of bleomycin （5 mg/kg body weight）. Normal saline （1 mL/kg body weight）was given intratracheally injection in the control group. There were 24 rats in each group, and 6 animals were separately killed on the 7 th, 14 th, 28t h and 35 th day after treated w...