期刊论文

INTERACTION OF ADENOVIRUS TYPE2 E1B 55 KILODALTON ONCOPROTEIN WITH HDAXX

中文

中国现代医学杂志

1005-8982

2003

13

2

10-12

10.3969/j.issn.1005-8982.2003.02.004

湖南省教委重点课题资助 （0 0A0 0 9）；

本校为第一机构

目的:研究腺病毒(adenovirus,Ad)2型E1B 55KD癌蛋白(Ad2 E1B 55KD)与人Daxx(human Daxx,hDaxx)的相互作用及作用部位.方法:利用共免疫沉淀反应研究Ad2/5E1B 55KD与hDaxx的结合反应,通过酵母双杂交体系测定两种蛋白质的相互作用.结果:酵母双杂交试验结果显示Ad2E1B 55KD通过C端58个氨基酸(amino acid,aa)与hDaxx结合并发生相互作用.共免疫沉淀反应和Western blot结果证实Ad2/5 E1B 55KD能在细胞内外与hDaxx直接结合.结论:Ad2E1B 55KD与hDaxx结合并发生相互作用.

Objective:To research the interaction of Adenovirus(Ad) type 2 E1B 55-kilodalton oncoprotein (Ad2 E1B 55kDa) with hDaxx ,and to study the direct binding of Ad E1B 55kDa with hDaxx in vivo or in vitro.Methods:The interaction of Ad2 EIB 55kDa with hDaxx was analyzed by the yeast two-hybrid assay. The direct binding of Ad2/5 EIB 55kDa with hDaxx was studied by coimmunoprecipitation reaction in vivo or in vitro. Results:Ad2 E1B 55kDa oncoprotein interacts with hDaxx by binding the C-terminus 58 amino acids(aa). Ad2/5 E1B 55kDa directly binds hDaxx in vivo or in vitro. Conclusi...