期刊论文

Huang, B.

中文

中国药理学与毒理学杂志

1000-3002

2020

33

8

587-594

10.3867/j.issn.1000-3002.2019.08.005

National Natural Science Foundation of China (U1803124)

本校为第一且通讯机构

目的 鉴定人支气管上皮HBE细胞中p53相关的γ射线诱导表达的长链非编码RNA(IncRNA)分子,为研究与p53关联的IncRNA分子在放射损伤反应如上皮间充质转化中的作用机制提供信息.方法 使用CRISPR-Cas9基因编辑技术构建p53敲除的人支气管上皮细胞(HBEp53-/-),Western印迹法检测P53表达水平,IncRNA芯片杂交分析IncRNA表达谱的改变,实时定量RT-PCR分析验证部分IncRNA分子的表达变化,生物信息学技术分析差异变化IncRNA的生物学功能.结果 与野生型HBE细胞相比,HBEp53-/-细胞在照射后有239条IncRNA出现上调表达,287条出现下调表达.上调和下调表达前5位IncRNA的差异表达变化经PCR得到验证.生物信息学GO分析表...

OBJECTIVE: To identify the differentially expressed long non-coding RNAs (IncRNAs) in γ-rays irradiated p53 knockout HBE (HBEp53-/-) cells, and to provide more baseline information for further investigation of p53 related IncRNAs in irradiation-induced injury, such as epithelial-mesenchymal transition in lung tissue epithelial cells. METHODS: HBEp53-/-cells were established with CRISPR-cas9. Western blotting was used to detect the knockout efficacy of P53 protein, while real time RT-PCR was used to verify the selected expressions of IncRNAs. B...