期刊论文

Qin, X.-P.

中文

中国药理学与毒理学杂志

1000-3002

2013

27

2

209-215

10.3867/j.issn.1000-3002.2013.02.015

国家自然科学基金（30572192） 国家自然科学基金（81173060）

本校为第一且通讯机构

目的探索受体活性修饰蛋白1(RAMP1)对血管紧张素II (Ang II)和(或)降钙素基因相关肽(CGRP)诱导的降钙素受体样受体(CRLR)在血管平滑肌细胞(VSMC)的表达和分布的影响,进一步揭示CGRP抑制VSMC增殖的机制。方法通过酶切、连接、转化等方法构建pCDNA3.1(+)-RAMP1真核表达载体并稳定转染至鼠源性血管平滑肌细胞株A10中,获得稳定高表达RAMP1的细胞系。无转染细胞、转染空载体〔pCDNA3.1(+)〕细胞和RAMP1高表达组细胞〔pCDNA3.1(+)-RAMP1〕分别用Ang II 100 nmol?L~(-1)、CGRP 100 nmol?L~(-1)和CGRP+Ang II处理24 h,用MTT法检测细胞存活;逆转录PCR、Western印迹和免疫荧光法分别检测CRLR mRNA含量、蛋...

OBJECTIVE: To investigate the effect of overexpression of receptor activity modifying protein 1 (RAMP1) on distribution of the calcitonin receptor like receptor (CRLR) in vascular smooth muscle cell (VSMC) in order to reveal the antiproliferative mechanism of calcitonin gene-related peptide(CGRP) for VSMC. METHODS: pCDNA3.1 (+)-RAMP1 eukaryon expression vector was successfully constructed by digestion, ligation, transform and transfected to the mouse VSMC cell line A10. After that the normal cells, pCDNA3.1 (+) cells and pCDNA3.1 (+)-RAMP1 cells were treated by Ang II, CGRP and CGRP + Ang II f...