期刊论文

Test research on working behaviour of water-proof layer in composite linings of highway tunnels

中文

中国公路学报

China Journal of Highway and Transport

1001-7372

2000

13

4

79-82

10.3321/j.issn:1001-7372.2000.04.018

本校为其他机构

目的灌注制备家猪、新西兰白兔、SD大鼠肾去细胞生物支架,探究三种肾支架对共培养种子细胞HEK的影响. 方法取健康成年家猪10头、新西兰白兔28只、SD大鼠28只,分别将取出的肾脏随机均等分为正常组和支架组,支架组由肾动脉依次灌入肝素、1% Triton X-100和1% SDS溶液完成去细胞化.两组肾分别作组织形态学鉴定并检测机械力学性质.去细胞支架作组织爬片与人胚肾上皮细胞共培养,观察细胞在支架爬片上的生长状况,免疫荧光检测人胚肾上皮细胞PCNA及DAPI表达量作灰度分析. 结果家猪、新西兰白兔、SD大鼠肾脏经灌注去细胞后HE核染色阴性, Masson染色显示胶原蛋白阳性,Collagen I和Collagen IV荧光染色阳性...

Through simulating tests on working behaviour of water proof layer in composite linings of highway tunnels, it is discovered that the roughness of the base for the water proof layer——the surface of concrete seriously affects the working condition of the layers during their long time service. Based on the tests,pressure deformation and shear deformation curves for the layers are established,and the shear strenthes are discussed as well. Meanwhile some suggestions for the design and construct...