期刊论文

Determination of 1-hydroxypyrene in human urine by fluorescence quenching

中文

中国卫生检验杂志

1004-8685

2020

30

20

2442-2445,2450

湖南省省级立项大学生研究性学习和创新性实验计划项目(477) 湖南省教育厅科学研究项目(1818C0423)。

本校为第一机构

目的 建立人尿中1-羟基芘(1-OHP)荧光猝灭法的测定方法.方法 在pH值为4.5的磷酸氢二钠-柠檬酸缓冲液中,1-OHP与鱼精蛋白-金纳米簇(PRT-AuNCs)反应,通过荧光猝灭程度(△F)得到标准曲线,对1-OHP进行定量.结果 在最佳实验条件下,测定值为1.03 nmol/L～62.80 nmol/L时,1-OHP与△F有良好的线性关系,线性回归方程为△F =577.91+ 17.89 c(10-9mol/L),R2为0.998 5,方法检出限为0.309 nmol/L,人尿样中1-OHP的加标回收率为94.39％～104.52％.结论 本法简便、快速、灵敏,所用仪器设备简单,易于推广,提高金纳米簇传感系统的灵敏度,可用于人尿中1-OHP的检测,为评价人体在多环芳烃(PAHs)下的暴露情况提供数据支...

Objective To establish a fluorescence quenching method for the determination of 1-hydroxypyrene( 1-OHP) in human urine.Methods 1-OHP reacted with protamine-gold nanocluster( PRT-Au NCs) in Na2 HPO4-citric acid buffer of p H 4.5.The standard curve was obtained by fluorescence quenching degree( ΔF),and the 1-OHP was quantified.Results Under the optimum experimental conditions,there was a good linearity between 1-OHP and ΔF when the determination was within 1.03 nmol/L-62.80 nmol/L.The linear regression equation was ΔF=577.91 + 17.89 c( 10-9 mo...