Objective: According to Stern - Volmer equation and double - reciprocal equation, the concentration of Znic (Ⅱ ) is denser, and the binding constant (K) are smaller. By studying the binding interaction between Zinc ( Ⅱ), BHA and BSA, the mechanism of the interaction among BHA, Zinc (Ⅱ ) and protein in organism, is furtherly discussed. Methods: The interaction between salicylic acid and bovine serum albumin, and the influence of Zinc ( Ⅱ ) on the system of salicylic acid and bovine serum albumin was studied under physiological co...