期刊论文

中文

中国卫生检验杂志

1004-8685

2005

15

2

160-161,173

10.3969/j.issn.1004-8685.2005.02.014

本校为第一机构

目的:提出检测Fenton反应产生羟自由基的新方法.方法:羟自由基与甲苯胺蓝发生氧化反应后,甲苯胺蓝的颜色发生变化,用分光光度计测定其ΔA值的变化,可间接测定羟自由基的生成量.通过对测定条件的研究,得到最佳测定方案.结果:甲苯胺蓝,FeSO4,H2O2与ΔA612 在一定范围内呈明显的量效关系.此外发现抗氧化剂药物甘露醇、抗坏血酸、硫脲与羟自由基清除率也呈明显的量效关系.结论:甲苯胺蓝光度法稳定性好,操作简便,测定快速,是一种简便的筛选抗羟自由基的清除剂和有效抗氧化剂药物的方法.

Objective:To develop a method for the determination of the hydroxyl free radical (·OH) produced by Fenton reaction. Methods:The color intensity of toluidine blue changed after the oxidation reaction and the change in absorbance, ΔA was measured photometrically and the amount of ·OH produced could be determined indirectly. The determination condition was studied and an optimum condition had been obtained. Results:The change in absorbance, ΔA 612 , was related quantitatively with the amount of toluidine blue and the reactants H 2O 2 and FeSO ...