期刊论文

中文

中国卫生检验杂志

1004-8685

2005

15

10

1161-1162+1211

10.3969/j.issn.1004-8685.2005.10.004

湖南省自然科学基金资助项目（03JJY3030）；

本校为第一机构

目的:建立一种灵敏简便的样品中8-OH-dG分析新方法.方法:在pH 9.0的R.B缓冲液中,吡啰红Y阳离子染料与8-OH-dG阴离子形成离子缔合物,导致体系的共振光散射增强.结果:在优化的实验条件下,体系的共振光散射强度随着8-OH-dG的加入在 339 nm 处急剧增强.共振光散射的增强(ΔIRLS)与8-OH-dG加入量成线性关系,据此建立8-OH-dG的共振光散射检测新方法,方法的线性范围为 50.0～226.4 ng/ml、检出限 15.2 ng/ml.结论:该方法快速、简便,已成功用于加标样品的分析.

Objective ： To develop a simple and sensitive method for determination of 8 - hydroxy - 2＇ - deoxygnanosine （ 8 - OH -dG）. Methods：In the pH 9. 0 buffer, the intensity of system＇s resonance light scattering （RLS） was significantly enhanced due to the formation of ion - association complex of 8 - hydroxy -2＇ - deoxyguanosine （8 - OH - dG） anion with pyronine Y cation. Results ： Under the optimal experimental conditions, a new RLS determination for 8 - OH - dG was developed based on the enhancement（ AIRes ） of the system＇s RLS inte...