期刊论文

A comparative study of regulation of the homing ability of mesenchymal stem cells by hypoxia and hydrogen peroxide pretreatment

中文

军事医学

1674-9960

2024

48

9

656-663

10.7644/j.issn.1674-9960.2024.09.003

82200295:国家自然科学基金 81971168:国家自然科学基金

本校为第一机构

目的 为提升间充质干细胞(MSC)归巢能力,通过比较不同氧浓度及过氧化氢(H2O2)环境下MSC中趋化因子(C-X-C基序)受体4(CXCR4)表达量,以确定促进CXCR4上调的最优条件.方法 分别在0.1％、1％、3％O2及H2O2(50 μmol/L)条件下培养MSC,并于不同时间点(3、6、12、24 h)收集细胞,通过Western印迹、实时定量PCR(qPCR)、免疫荧光染色方法测定MSC中CXCR4的表达量;用CCK-8实验、划痕实验、Transwell迁移实验检测MSC经不同处理后细胞活力及趋化能力的变化.结果 经低氧及H2O2处理后,发现MSC中CXCR4均有一定程度的上调,结合蛋白和mRNA的表达情况,在3％O2条件下处理时间为6～12 h时有相对较高的表达水平,H2O2则在...

Objective To determine the optimal conditions for CXCR4 upregulation by comparing the expression levels of chemokine(C-X-C motif)receptor 4(CXCR4)in MSCs cultured with varying concentrations of oxygen and hydrogen peroxide(H2O2).Methods MSCs were cultured with 0.1％,1％,or 3％O2 and 50 μmol/L H2O2 for different lengths of time(3,6,12,and 24 h).The mRNA and protein expressions of CXCR4 in MSCs were measured by real-time quantitative PCR(qPCR),Western blotting,and immunofluorescence staining.The viability and chemotactic ability of MSCs were mea...