期刊论文

Identification and analysis of exosomal miRNAs in human umbilical vein endothelial cells irradiated by ~(60)Co γ-rays

中文

军事医学

1674-9960

2017

41

5

367-372

10.7644/j.issn.1674-9960.2017.05.009

国家自然科学基金重点资助项目（81530085）； 湖南省自然科学基金资助项目（2016JJ2115）；

本校为第一机构

目的利用微小RNA(miRNA)芯片和生物信息学技术,研究受照射人脐静脉血管内皮细胞(HUVEC)产生的外泌体miRNA组分的变化,为揭示血管组织放射损伤及其旁效应机制提供新的线索。方法超高速离心法收集对照组和4 Gy剂量照射组的HUVEC外泌体,运用电镜及Western印迹技术对外泌体进行鉴定,miRNA芯片技术分析细胞内和外泌体中miRNA表达谱,qRT-PCR法验证部分差异miRNA,通过miRDB和TargetScan预测差异miRNA的靶基因,DAVID、KEGG等在线工具进行生物信息学分析。结果HUVEC经4 Gy照射后外泌体miRNA与对照组相比,照后0.5 h共鉴定到18个发生表达变化的miRNA分子,5个表达上调,13个表达下调;照后2 h鉴定出16个表达上...

Objective To study the changes in miRNAs expression in the exosomes of human umbilical vein endothelial cells（HUVECs） after 60Co γ-rays expose using microRNA（miRNA） chips and bioinformatics techniques so as to provide new clues to the mechanism of radiation-induced vascular tissue injury and its bystander effects. Methods HUVECs exosomes were collected in the control and 4 Gy irradiated cells by ultra-high-speed centrifugation, and further confirmed using transmission electron microscopy （TEM） and Western blotting of exosomes biomarkers,...