期刊论文

Application of DNA functionalized gold nanoparticles in detection of Chlamydia trachomatis

Chen, L.-L.

中文

中国生物制品学杂志

1004-5503

2019

32

4

428-433

10.13200/j.cnki.cjb.002565

国家自然科学基金面上项目(81572011) 湖南省自然科学基金青年项目(2016JJ3103) 湖南省研究生科研创新项目(CX2018B626).

本校为第一且通讯机构

目的建立一种基于DNA功能化金纳米颗粒检测沙眼衣原体的新方法。方法分析沙眼衣原体(Chlamydia trachomatis,Ct)TrpB保守基因序列,设计2条特异性寡核苷酸链,一条进行巯基修饰后与柠檬酸钠还原法制备的金纳米颗粒结合得到信号探针,另一条用生物素标记作为捕获探针。制备的靶序列与两种探针在包被链霉亲和素的酶标板内形成牢固的带有金纳米颗粒的核酸复合物,加入银染液后,经酶标仪读取630 nm处A值或肉眼观察96孔板底灰度。对建立的检测体系进行特异性和灵敏度验证。用建立的检测体系及荧光定量PCR法对可疑临床沙眼衣原体样本进行检测。结果制备的金纳米颗粒经透射电子显微镜(transmission electro...

Objective: To establish a new method for detection of Chlamydia trachomatis with DNA functionalized gold nanoparticles. Methods: Two specific oligonucleotide chains were designed according to the TrpB gene sequence of C. trachomatis, of which one was combined with gold nanoparticles obtained by sodium citrate reduction method after thiol modification and used as the signal probe, while the another one was labeled with biotin and used as the capture probe. The prepared target sequence and the two probes formed a strong complex with gold nanopart...