The purpose of this study was to construct and purify a fusion expression and purification of the protein CFP10-ESAT6(CE)of Mycobacterium tuberculosis(MTB)and then preliminarily evaluated its immune effect.The recombinant plasmid pET32a(+)-CE was constructed,with which CE was expressed in E.coli BL21(DE3)and purified by Ni-NTA chromatography.BALB/c mice were immunized with CE adjuvanted with aluminum for three times,the dosage of CE was 50μg per mouse per time.Serum IgG antibodies were determined by ELISA;cytokines were detected by ELISpot and...
