Identification of non-tuberculosis mycobacteria species of clinical isolates from patients clinicallydiagnosed with tuberculosis in Fujian Province,China

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成果类型：

期刊论文

作者：

刘海灿;黄明翔;蒋毅;连璐璐;赵秀芹;...

作者机构：

National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Diseases Prevention and Control

[刘海灿; 蒋毅; 连璐璐; 赵秀芹; 万康林] Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing, 102206

Fuzhou Pulmonary Hospital, Fuzhou, 350008

Pathogenic Biology Institute,University of South China, Hengyang, 421001

[刘海灿; 蒋毅; 连璐璐; 赵秀芹; 万康林] National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Diseases Prevention and Control

语种：

中文

关键词：

菌种鉴定;分枝杆菌;核酸测序

关键词(英文)：

identification;Mycobacterium;PRA;DNA sequencing

期刊：

中国人兽共患病学报

ISSN：

1002-2694

年：

2017

卷：

期：

页码：

389-397

DOI：

10.3969/j.issn.1002-2694.2017.05.002

基金类别：

Funded by the project of National Key Program for Infectious Diseases of China

机构署名：

本校为第一机构

院系归属：

医学院

摘要：

目的对2005-2011年间,福建省福州市胸科医院分离获得的非结核分枝杆菌临床菌株进行菌种鉴定。方法分别使用传统鉴别培养基法和多位点PCR、hsp65-PRA与rpoB-PRA,以及hsp65、rpoB、16srRNA和ITS基因测序等分子生物学方法,对全部菌株进行菌种鉴定。结果 450株被初步鉴定为非结核分枝杆菌的临床分离菌株中,有45株被鉴定为结核分枝杆菌,其余405株被鉴定为23种不同的非结核分枝杆菌和1株支气管戈登菌。结论在该地区有多种非结核分枝杆菌传播和流行,其中,6种非结核分枝杆菌和支气管戈登菌为在福建省内首次发现。

摘要(英文)：

Some species of non-tuberculosis mycobacteria(NTM)are important causes of human disease and infections.The purpose of this study was to describe the NTM species of clinical isolates from patients with tuberculosis in Fujian,China.The clinical NTM strains isolated from the patients clinically diagnosed with tuberculosis collected from 2005to 2011in Fuzhou Pulmonary Hospital,Fujian Province,and were identified to species level using conventional and molecular methods including multi-loci PCR,PRA-hsp65and PRA-rpoBand sequencing for hsp65,rpoB,16S rRNA and ITS.A total of 450pre-identified NTM isol...

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Identification of non-tuberculosis mycobacteria species of clinical isolates from patients clinicallydiagnosed with tuberculosis in Fujian Province,China

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