期刊论文

Bioinformatic analysis of Chlamydophila pneumoniae Cpn0797 protein and the preparation of its monoclonal antibody

中文

中国人兽共患病学报

1002-2694

2012

28

2

103-107

10.3969/j.issn.1002-2694.2012.02.002

国家自然科学基金（No.81072417）； 湖南省卫生厅科研课题（B2007093）联合资助；

本校为第一机构

目的 生物信息学分析肺炎嗜衣原体Cpn0797蛋白的结构,制备特异性的抗Cpn0797蛋白的单克隆抗体.方法 用ProtParam、SignalP、NPS@、和PSORT等软件对Cpn0797蛋白的理化参数、信号肽、二级结构、蛋白亚细胞定位进行分析;原核表达纯化GST-Cpn0797融合蛋白,以其为免疫原免疫BALB/c小鼠.杂交瘤技术制备单克隆抗体,采用间接免疫荧光法鉴定单克隆抗体的亚类和特异性.结果 生物信息学分析表明Cpn0797蛋白二级结构以无规则卷曲为主;成功地建立了能稳定分泌抗Cpn0797蛋白的单克隆抗体杂交瘤细胞株,单克隆抗体能特异性的识别Cpn0797内源性蛋白.结论成功制备特异性的Cpn0797单克隆抗体,为进一步探究Cpn0797蛋...

In order to analyze the protein structure of Chlamydophila pneurnoniae Cpn0797 protein and to prepare spe cific monoclonaI antibodies （mAb） against Cpn0797 protein, ProtParam, SignalP, NPS@ and PSORT software packages were used to predict the physical and chemical properties, signal peptide, secondary structure and protein localization sites in cells according to the amino acid sequence of Cpn0797. The gene cpn0797 was cloned into the prokaryotic expression vector pGEX6p-2, and expressed in E. coli and then purified. The mAbs against Cpn0797 ...