期刊论文

中文

中国人兽共患病学报

1002-2694

2004

20

8

690-694

10.3969/j.issn.1002-2694.2004.08.010

湖南省自然科学基金 （ 0 1jjy2 114 ）； 湖南省卫生厅科研基金（ 0 0 0 2 2 ）； 国家教育部优秀骨干教师基金 （教技司 2 0 0 0 -65 ）；

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目的探讨H.pylori对HepG2 c-fos基因表达的影响.方法将H.pylori与HepG2共同培养1、3、6、12和24h,采用RT-PCR和Western blot方法检测不同作用时相细胞的c-fos mRNA和蛋白表达.结果cgA+ H.pylori作用HepG2后c-fos呈短暂一过性表达升高,c-fos mRNA、蛋白质在H.pylori感染后1h开始增加,6h达高峰,表达水平分别为对照组的2.5倍和1.6倍(P＜0.01).12h开始下降,24h基本恢复到刺激前水平.而cgA-H.pylori及空肠弯曲菌与HepG2细胞共培养后,未见该基因的表达升高.结论cag+AH.pylori可诱导HepG2 c-fos 基因表达升高,其在肝癌发生中的作用有待进一步研究.

To investigate the expression of c-fos by H.pylori in HepG2.H.pylori was co-cultured with HepG2 for 1、3,6、12 and 24h.RT-PCR and Western blot were performed to detect the expression of c-fos mRNA and protein respectively.The results showed that after 1h of cag +_A H.pylori treatment,the level of c-fos mRNA and protein began to increase,and the peak occurred at 6h after stimulation.The expression of c-fos mRNA and protein were 2.5 and 1.6 times of the controls respectively.The expression of c-fos decreased gradually at 12h and turned to normal ...