期刊论文

中文

中国人兽共患病学报

1002-2694

2004

20

4

299-302,314

10.3969/j.issn.1002-2694.2004.04.008

湖南省教育厅科研项目

本校为第一机构

目的体外扩增日本血吸虫中国大陆株粘蛋白样蛋白抗原的部分基因序列,构建日本血吸虫pcDNA3.1(+)/MLP核酸疫苗(SJP),免疫新西兰家兔,观察它在肌肉组织中的表达、及其对家兔的保护性免疫作用.方法分子克隆常规操作构建SJP疫苗,免疫家兔,家兔肌肉组织体外扩增查MLP基因,并通过免疫组化观察重组蛋白在局部肌组织中的表达;做血清抗体及细胞因子分析,计算减虫率及减卵率.结果经酶切、PCR及测序鉴定表明所构建的SJP中含有目的基因序列;免疫家兔8周后可以在免疫家兔肌肉中检测到MLP基因,免疫组化结果阳性;可诱导IgA、IgG1及INF-γ变化,减虫率为24.10%,减卵率为28.10%.结论成功地构建了含目的基因的核酸疫...

To construct the nucleic acid vaccine pcDNA3.1(+)/MLP (SJP) by amplifying the part of genes coding for mucin-like protein of Schistosoma japanica (SjMLP) and by cloning the fragment into eukaryotie expression vector pcDNA3.1(+).New Zealand rabbits were vaccinated with SJP,and the MLP gene was detected in the muscle tissue of immunized rabbits by PCR.The expression of the recombinant protein in local muscle tissue was detected with immunohisto-biochemical methods.At the same time,serum antibody titers,cytokine analysis and reduction rates of adu...