期刊论文

Tan, L.M.

中文

中华血液学杂志

0253-2727

2004

25

5

273-276

10.3760/j:issn:0253-2727.2004.05.005

国家自然科学基金资助项目 （ 3 0 2 70 684）； 湖南省杰出中青年专家专项基金资助项目 （ 0 2JJYB0 0 4）； 湖南省自然科学基金资助项目 （ 0 3JJY3 0 2 8）；

本校为第一机构

目的探讨二烯丙基二硫(DADS)诱导HL-60细胞G2/M期细胞生长阻滞的分子机制.方法用不同浓度的DADS处理HL-60细胞,分别作用0,6,12,24,48 h后用MTT法测定细胞增殖活性;用流式细胞术和有丝分裂指数测定细胞增殖周期;用Western blot检测p38丝裂原活化蛋白(p38 MAPK)及Cdc25B和Cdc2激酶的表达和磷酸化水平;用RT-PCR检测p38 MAPK mRNA的表达.结果 DADS抑制HL-60细胞增殖呈浓度依赖性,且DADS(20 μmol/L)与ATRA(10 nmol/L)对HL-60细胞增殖活性影响相近(P>0.05);DADS 20 μmol/L作用HL-60细胞12 h后能引起G2/M期细胞百分数增高并达到最大值,而此时有丝分裂指数明显下降(P

OBJECTIVE: To explore the molecular mechanisms of G(2)/M checkpoint initiated by diallyl disulfide (DADS) in HL-60 cells. METHODS: Cell viability was determined by MTT assay. Cell cycle was assayed by flow cytometry. The expression of phospho-p38, Cdc25B and Cdc2, and p38 mRNA were measured by Western blotting and RT-PCR, respectively. RESULTS: After treatment with DADS at 5 - 160 micro mol/L for 0 - 72 h, the growth of HL-60 cells were suppressed in a concentration-dependent manner and the inhibitory effect of DADS (20 micro mol/L) was similar to that of ATRA (10 nmol/L) (P > 0.05). Incubatio...