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Site-specific incorporation of 19F-nulcei at protein C-terminus to probe allosteric conformational transitions of metalloproteins

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成果类型:
期刊论文
作者:
Liu, Xichun*;Guo, Pengfei;Yu, Qiufan;Gao, Shu-Qin;Yuan, Hong;...
通讯作者:
Liu, Xichun;Lin, YW
作者机构:
[Guo, Pengfei; Lin, Ying-Wu; Yu, Qiufan; Liu, Xichun; Liu, XC] Univ South China, Sch Chem & Chem Engn, Hengyang, Peoples R China.
[Gao, Shu-Qin; Lin, Ying-Wu] Univ South China, Hengyang Med Sch, Key Lab Prot Struct & Funct Univ Hunan Prov, Hengyang, Peoples R China.
[Yuan, Hong; Tan, Xiangshi] Fudan Univ, Dept Chem, Shanghai, Peoples R China.
[Yuan, Hong; Tan, Xiangshi] Fudan Univ, Inst Biomed Sci, Shanghai, Peoples R China.
通讯机构:
[Lin, YW ; Liu, XC] U
Univ South China, Sch Chem & Chem Engn, Hengyang, Peoples R China.
Univ South China, Hengyang Med Sch, Key Lab Prot Struct & Funct Univ Hunan Prov, Hengyang, Peoples R China.
语种:
英文
期刊:
Communications biology
ISSN:
2399-3642
年:
2024
卷:
7
期:
1
页码:
1613
基金类别:
the National Natural Science Foundation of China (22107046 and 21977042), Provincial Natural Science Foundation of Hunan (2024JJ6378). [22107046, 21977042]; National Natural Science Foundation of China [2024JJ6378]; Provincial Natural Science Foundation of Hunan
机构署名:
本校为第一且通讯机构
院系归属:
化学化工学院
摘要:
Allosteric conformational change is an important paradigm in the regulation of protein function, which is typically triggered by the binding of small cofactors, metal ions or protein partners. Here, we found those conformational transitions can be effectively monitored by 19F NMR, facilitated by a site-specific 19F incorporation strategy at the protein C-terminus using asparaginyl endopeptidase (AEP). Three case studies show that C-terminal 19F-nuclei can reveal protein dynamics not only adjacent but also distal to C-terminus, including those occurring in a hemoprotein neuroglobin (Ngb), calmo...

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