期刊论文

The method of simultaneous determination of 11 synthetic pigments including acid orange II in meat products

中文

中国食品卫生杂志

1004-8456

2014

26

4

372-376

10.13590/j.cjfh.2014.04.017

武汉市卫生局资助课题（WG11B02）；

本校为第一机构

目的 构建同步测定肉制品中柠檬黄、新红、苋菜红、胭脂红、日落黄、诱惑红、红色2G、亮蓝、酸性红、赤藓红、酸性橙Ⅱ等11种合成色素的高效液相色谱法(HPLC),对市售肉制品中色素进行监测.方法 采用固相萃取技术,用乙醇氨水水溶液提取样品中色素并经WAX小柱(150 mg,30μm)净化,采用高效液相色谱-二极管阵列检测器(HPLC-PDA)检测.结果 HPLC法测定11种色素在1.00～20.0 μg/ml范围内有较好的线性关系,r均＞0.999,方法检出限为0.02 ～0.06 mg/kg,样品加标平均回收率为81.1％ ～ 100.8％,RSD为1.3％～4.9％(n=6).用本方法监测259份市售肉制品,不同色素的检出率为0％ ～ 22.0％.结论 方法具有较高的选...

Objective To establish a high performance liquid chromatography （HPLC） method of simultaneous detection of 11 kinds of artificial pigments including Tartrazine, New red, Amaranth, Carmine, Sunset yellow, Allura Red, Red 2G, Acid blue 9, Azorubine, Erythrosine and Acid orange Ⅱ, et. al. Methods The target compounds in food samples were extracted by ethanol ammonia and purified by WAX cardge. The extracts were determined by a high performance liquid chromatography-diode array detector. Results The results showed that there were good linear rel...