期刊论文

Tang, S. Y.

英文

Molecular Biology

0026-8933

2014

48

4

594-598

10.1134/S0026893314040165

This study was supported by the construct program of the key discipline in Hunan Province [Hunan Pro vincial Education Department document (Approval number: 2012 1)], and Hunan Provincial Key Labo ratory for Special Pathogens Prevention and Control [Hunan Provincial Science and Technology Department document (Approval number: 2014 5) and Hunan Pro vincial Education Department document (Approval number: 2012 312)], and by grants of the National Nat ural Science Foundation of China (No. 30973402), the Educational Committee of Hunan Province (No. 11A102) and Science and Technology Department of Hunan Province (No. 2013FJ6068).

本校为第一且通讯机构

The aim of the study was to explore the interactions of human papilloma virus 16 (HPV16) E2 protein and Daxx. The localization or co-localization of PML and E2 with Daxx in Caski cells was observed by indirect immunofluorescence. The interaction of E2 and Daxx was analyzed by co-immunoprecipitation, Western-blot and yeast-two hybrid assays. In Caski cells the fluorescence of Daxx and PML was mainly distributed in the cytoplasm or nucleus, respectively, and in the align image their signals did not overlap. However, when the red signal of HPV16 E...