期刊论文

Tang, S. Y.

英文

Molecular Biology

0026-8933

2014

48

4

594-598

10.1134/S0026893314040165

construct program of the key discipline in Hunan Province [Hunan Provincial Education Department] [2012-1]; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control [Hunan Provincial Science and Technology Department] [2014-5]; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control [Hunan Provincial Education Department] [2012-312]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30973402]; Educational Committee of Hunan Province [11A102]; Science and Technology Department of Hunan Province [2013FJ6068]

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The aim of the study was to explore the interactions of human papilloma virus 16 (HPV16) E2 protein and Daxx. The localization or co-localization of PML and E2 with Daxx in Caski cells was observed by indirect immunofluorescence. The interaction of E2 and Daxx was analyzed by co-immunoprecipitation, Western-blot and yeast-two hybrid assays. In Caski cells the fluorescence of Daxx and PML was mainly distributed in the cytoplasm or nucleus, respectively, and in the align image their signals did not overlap. However, when the red signal of HPV16 E2 and the green signal of Daxx in the cytoplasm of...