Quantum dots-based double imaging combined with organic dye imaging to establish an automatic computerized method for cancer Ki67 measurement

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成果类型：

期刊论文

作者：

Wang, Lin-Wei;Qu, Ai-Ping;Liu, Wen-Lou;Chen, Jia-Mei;Yuan, Jing-Ping;...

通讯作者：

Li, Yan;Liu, Juan

作者机构：

[Wang, Lin-Wei; Li, Yan] Capital Med Univ, Beijing Shijitan Hosp, Dept Surg Oncol, Beijing 100038, Peoples R China.

[Wang, Lin-Wei; Wu, Han; Liu, Wen-Lou; Li, Yan; Chen, Jia-Mei] Wuhan Univ, Zhongnan Hosp, Dept Oncol, Wuhan 430071, Peoples R China.

[Qu, Ai-Ping] Univ South China, Sch Comp Sci & Technol, Hengyang 421001, Peoples R China.

[Yuan, Jing-Ping] Wuhan Univ, Renmin Hosp, Dept Pathol, Wuhan 430060, Peoples R China.

[Liu, Juan] Wuhan Univ, Sch Comp, Wuhan 430072, Peoples R China.

通讯机构：

[Li, Yan] C

[Li, Yan; Liu, Juan] W

Capital Med Univ, Beijing Shijitan Hosp, Dept Surg Oncol, Beijing 100038, Peoples R China.

Wuhan Univ, Zhongnan Hosp, Dept Oncol, Wuhan 430071, Peoples R China.

Wuhan Univ, Sch Comp, Wuhan 430072, Peoples R China.

语种：

英文

期刊：

Scientific Reports

ISSN：

2045-2322

年：

2016

卷：

期：

页码：

20564

DOI：

10.1038/srep20564

基金类别：

Key Project of the National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81230031/H18]; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81201196/H18]; Program for New Century Excellent Talents in UniversitiesProgram for New Century Excellent Talents in University (NCET) [NCET-10-0644]; Fundamental Research Fund for the Central Universities [303274028]

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本校为其他机构

院系归属：

计算机科学与技术学院

摘要：

As a widely used proliferative marker, Ki67 has important impacts on cancer prognosis, especially for breast cancer (BC). However, variations in analytical practice make it difficult for pathologists to manually measure Ki67 index. This study is to establish quantum dots (QDs)-based double imaging of nuclear Ki67 as red signal by QDs-655, cytoplasmic cytokeratin (CK) as yellow signal by QDs-585, and organic dye imaging of cell nucleus as blue signal by 4',6-diamidino-2-phenylindole (DAPI), and to develop a computer-aided automatic method for Ki67 index measurement. The newly developed automati...

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Quantum dots-based double imaging combined with organic dye imaging to establish an automatic computerized method for cancer Ki67 measurement

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