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The antioxidant activities of Mycoplasma pneumoniae MPN662; [肺炎支原体MPN662的抗氧化活性研究]

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成果类型:
期刊论文
作者:
He;Guiting (58146161000);Li;Tingting (57226117610);Zhang;...
作者机构:
Key Laboratory of Prevention and Control of Special Pathogens in Hunan Provincen, Institute of Pathogen Biology, Hengyang Medical College, University of South China, Hengyang, 421001, China
Major in Medical Laboratory Technology, Chuanshan College, University of South China, Level 2020, Hengyang, 421001, China
Blood Transfusion Department, the Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang, 421001, China
语种:
中文
关键词:
cysteine;fluorescent dye;hydrogen peroxide;membrane protein;reactive oxygen metabolite;serine;superoxide dismutase;tert butyl hydroperoxide;antioxidant activity;Article;bacterial gene;cell stimulation;concentration (parameter);dose response;enzyme activity;enzyme synthesis;gene expression level;gene function;genetic association;genetic transcription;human;human cell;mpn662 gene;Mycoplasma pneumoniae;nonhuman;protein analysis;protein expression;protein expression level;protein purification;regulatory mechanism;reverse transcription polymerase chain reaction;structure analysis;THP-1 cell line;Western blotting
期刊:
中华微生物学和免疫学杂志
ISSN:
0254-5101
年:
2024
卷:
44
期:
10
页码:
853-859
基金类别:
National Natural Science Foundation of China, NSFC, (31970177) National Natural Science Foundation of China, NSFC Natural Science Foundation of Hunan Province, (2022JJ30543) Natural Science Foundation of Hunan Province College Students′ Innovative Entrepreneurial Training Plan Program, (202212650009, S202212650007)
机构署名:
本校为第一且通讯机构
院系归属:
医学院
船山学院
摘要:
Objective To investigate the antioxidant function of Mycoplasma pneumoniae MPN662 and analyze the key active sites, and to explore the role of MPN662 in the regulation of the production of reactive oxygen species (ROS) and superoxide dismutase (SOD) in THP-1 cells. Methods pET28a(+ )-mpn662, recombinant mutant plasmids pET28a(+ )-mpn662-Ser66 (the 66th Cys was mutated to Ser) and pET28a(+ )-mpn662-Ala66 (the 66th Cys was mutated to Ala) were constructed, recombinant proteins rMPN662, rMPN662-Ser66 and rMPN662-Ala66 were expressed, identified, a...

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