肺炎衣原体假定蛋白Cpn0423通过NOD2信号途径诱导炎症反应的作用机制

首页 > 成果 > 详情

认领

导出

Link by 中国知网学术期刊 Link by 万方学术期刊

反馈

作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

论文标题(英文)：

Contribution of NOD2 signal pathway to Chlamydia pneumonia Cpn0423-induced inflammatory response

作者：

李佳艳;罗良贤;周洲;周安文;何蓓;...

通讯作者：

Chen, H.

作者机构：

[周安文; 罗良贤; 李佳艳] Chlamydia R&D Center of Chenzhou, Chenzhou NO.1 People's Hospital, Chenzhou, 423000, China

[何蓓; 周洲; 吴移谋] Institute of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, 421001, China

Department of Laboratory Medicine, Chenzhou Hospital Affiliated to Southern Medical University, Chenzhou, 423000, China

[陈虹亮] Chlamydia R&D Center of Chenzhou, Chenzhou NO.1 People's Hospital, Chenzhou, 423000, China, Institute of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, 421001, China, Department of Laboratory Medicine, Chenzhou Hospital Affiliated to Southern Medical University, Chenzhou, 423000, China

[李胜涛; 江扬华] Chlamydia R&D Center of Chenzhou, Chenzhou NO.1 People's Hospital, Chenzhou, 423000, China, Department of Laboratory Medicine, Chenzhou Hospital Affiliated to Southern Medical University, Chenzhou, 423000, China

通讯机构：

[Chen, H.] C

Chlamydia R&D Center of Chenzhou, China

语种：

中文

关键词：

肺炎衣原体;炎症反应

关键词(英文)：

Cpn0423;NOD2

期刊：

中华微生物学和免疫学杂志

ISSN：

0254-5101

年：

2020

卷：

期：

页码：

690-696

DOI：

10.3760/cma.j.cn112309-20200413-00188

基金类别：

0.001). Moreover, Cpn0423-induced production of MIP-2 [(920. 5±99. 1) pg/ml vs (130. 1±11. 5) pg/ml, P<0.001] and IL-6 [(266.2±58.4) pg/ml vs (165.7±21.5) pg/ml, P<0.001] in BMDMs were decreasedfollowingNOD2-siRNApre-treatment. Cpn0423DNAwasdetectedintheBAlFof83.3% (10/ 12) of Cpn-positive cases, but not in Cpn-negative cases. Conclusions　 Cpn0423 induced inflammatory response in host cells through NOD2 pathway, which was closely related to the chronic inflammatory injury caused by Cpn. 【 Key words 】　 Chlamydia pneumonia; Cpn0423; NOD2; Inflammatory response Fund programs: National Natural Science Foundation of China (81802022); Planning Project of Hunan Provincial Science and Technology Department (2018SK50309); Chenzhou Science and Technology Bureau (yfzx201908, zdyf201941) DOI: 10. 3760 / cma. j. cn112309-20200413-00188

机构署名：

本校为第一机构

院系归属：

医学院

摘要：

目的:了解肺炎衣原体( Chlamydia pneumoniae，Cpn)假定蛋白Cpn0423的生物学活性及其诱导宿主细胞炎症反应的作用途径。方法:生物信息学软件初步分析Cpn0423的生物学特性，激光共聚焦显微技术检测核苷酸结合寡聚化结构域样受体2(nucleotide-binding oligomerization domain-like receptor 2, NOD2)在骨髓源性的巨噬细胞(bone marrow-derived macrophages, BMDMs)中的亚细胞定位。采用NOD2-siRNA干扰试验检测其抑制BMDMs细胞中NOD2的表达水平，ELISA法检测Cpn0423诱导BMDMs细胞分泌的巨噬细胞炎性蛋白2(MIP-2)和IL-6，PCR法检测Cpn阳性患者支气管肺泡灌洗液(bronchoalveolar lavage fluid，BA...

摘要(英文)：

Objective: To understand and determine the biological properties of Chlamydia pneumonia (Cpn) hypothetical protein Cpn0423 and the mechanisms of which involved in Cpn0423-induced inflammatory response. Methods: The biological properties of Cpn0423 gene were analyzed using bioinformatic software. The subcellular localization of nucleotide-binding oligomerization domain-like receptor 2 (NOD2) in bone marrow-derived macrophages (BMDMs) was detected by confocal microscope. NOD2-siRNA was used to inhibit the expression of NOD2 at mRNA level. Cpn0423...

反馈

产权有误：本人成果被他人认领

数据有误：数据基本信息有误

归属有误：成果的院系归属、机构署名归属有误

其他原因：

验证码：

看不清楚，换一个

确定

取消

成果认领

标题：

用户	作者	通讯作者	--
	请选择	请选择	--

确定

取消

肺炎衣原体假定蛋白Cpn0423通过NOD2信号途径诱导炎症反应的作用机制

反馈

成果认领

提示

该栏目需要登录且有访问权限才可以访问