期刊论文

IL-10 promotes Chlamydia muridarum infection in mice through inhibiting IFN-γ and IL-2 expression

He, Q.

中文

中华微生物学和免疫学杂志

0254-5101

2019

39

2

126-130

10.3760/cma.j.issn.0254-5101.2019.02.009

National Natural Science Foundation of Hunan Province of China (2018JJ2326) National Natural Science Foundation of China (81202323)

本校为第一且通讯机构

目的初步探讨IL-10促进鼠衣原体感染的可能分子机制。方法鼠衣原体经灌胃或子宫内感染C57BL/6野生型和IL-10~(-/-)小鼠后,采用间接免疫荧光试验检测肠道和生殖道内鼠衣原体的生长情况;输卵管积水评分评估生殖道疾病严重程度;酶联免疫吸附试验(ELISA)检测血液中IFN-γ和IL-2的表达水平。结果与野生型小鼠相比,IL-10~(-/-)小鼠的肠道和生殖道均具有较强的衣原体清除能力,同时细胞因子IFN-γ和IL-2的表达显著性增加。此外,野生型小鼠表现出更严重的输卵管病理损伤。结论IL-10通过抑制IFN-γ和IL-2的表达使宿主清除衣原体能力下降,从而促进衣原体在小鼠体内生长,加重输卵管积水的发病率。

Objective: To study the possible molecular mechanism of IL-10 in promoting Chlamydia muridarum infection in mice. Methods: C57BL/6 wild-type and IL-10 gene knockout (IL-10-/-) mice were infected with Chlamydia muridarum. Indirect immunofluorescence assay was used to detect the growth of Chlamydia muridarum in the intestinal and genital tracts. The severity of genital diseases was assessed by hydrosalpinx scoring. Expression of IFN-γ and IL-2 in blood was measured by ELISA. Results: Compared with the wild-type group, Chlamydia clearance in the ...