期刊论文

Zhu, C.

中文

中华微生物学和免疫学杂志

0254-5101

2017

37

11

862-868

10.3760/cma.j.issn.0254-5101.2017.11.010

国家自然科学基金

本校为第一且通讯机构

目的了解2013—2016年湖南省衡阳地区儿童肺炎支原体(Mp)感染情况,用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)、巢式PCR(nPCR)和快速循环PCR(Rapid-Cycle PCR)对Mp p1基因进行分型研究。方法收集2013—2016年衡阳市4家三甲医院儿童急性呼吸道感染咽拭子标本,PCR扩增16S rRNA基因鉴定Mp临床株。用PCR-RFLP、nPGR和Rapid-Cycle PCR对临床株进行pl基因分型,分析2013—2016年衡阳地区Mp的流行情况。结果从984例咽拭子标本中共鉴定出109株Mp菌株。PCR-RFLP和nPCR对109例Mp菌株分型的敏感性为100% (109/109)。 Rapid-Cycle PCR分型法的敏感性为98.17% (107/109),3种方法基因分型的特异性均为100%...

Objective: To investigate the prevalence of Mycoplasma pneumoniae (Mp) infection in children in Hengyang from 2013 to 2016 and to analyze the p1 genotypes of the isolated Mp strains by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), nested polymerase chain reaction (nPCR) and rapid-cycle polymerase chain reaction (Rapid-Cycle PCR). Methods: Throat swab samples of children with acute respiratory tract infection were collected from four hospitals in Hengyang, Hunan Province from 2013 to 2016. Mp strains in the...