期刊论文

Wu, Y.

中文

中华微生物学和免疫学杂志

0254-5101

2015

35

12

921-925

10.3760/cma.j.issn.0254-5101.2015.12.012

国家自然科学基金

本校为第一且通讯机构

目的构建糖尿病合并梅毒兔感染模型,动态观察肾脏损伤情况及初步探讨其机制。方法采用四氧嘧啶诱导法构建单独糖尿病组(DM组),采用四氧嘧啶诱导糖尿病再接种梅毒螺旋体致其感染构建合并梅毒组(H组),另设单独梅毒组(T组)和正常对照组(C组),动态观察各组兔肾脏损伤改变情况并初步研究炎症相关硫氧还蛋白相互作用蛋白(thioredoxin-interacting protein, TXNIP)的表达情况。结果成功构建了各组兔感染模型,自第8周起,与T组和DM组相比,H组兔随实验时间增长尿素氮及肌酐明显升高;与C组比较,各实验组肾脏组织有明显损伤,且H组损伤程度明显比单独疾病组T组和DM组严重;免疫组化结果显示,各实验组肾脏TXNIP...

Objective: To establish a rabbit model of diabetes mellitus in combination with syphilis for further monitoring the dynamic changes in rabbits' kidneys and investigating the possible pathogenic mechanism. Methods: Twenty-four male rabbits were divided into four groups including diabetes group (group DM, n=8), syphilis group (group T, n=5), diabetes mellitus in combination with syphilis group (group H, n=8) and control group (group C, n=3). The rabbit models for groups DM, T and H were respectively established by injecting rabbits with alloxan, ...