期刊论文

Wu, Y.

中文

中华微生物学和免疫学杂志

0254-5101

2014

34

4

289-293

10.3760/cma.j.issn.0254-5101.2014.04.007

国家自然科学基金（31000091）；

本校为第一且通讯机构

目的：探讨支原体巨噬细胞活化脂肽2（ MALP-2）能否诱导人单核细胞系THP-1产生血红素氧合酶-1（HO-1），并探讨其可能的调控机制。方法体外培养THP-1细胞，根据实验目的，用不同浓度的MALP-2作用12 h，或一定浓度的MALP-2作用不同时间后，real-time PCR和Western blot分别检测HO-1 mRNA和蛋白的表达；比色法分析 HO-1酶活性情况；采用丝裂原活化蛋白激酶（ MAPKs）特异性抑制剂（30μmol/L SB203580、PD98059和SP600125）预处理THP-1细胞30 min后加入5．0 ng/ml MALP-2共孵育细胞，Western blot检测HO-1的蛋白表达。提取MALP-2处理后的核蛋白及胞质蛋白，Western blot检测转录因子NF-E2相关因子...

Objective: To investigate whether macrophage-activating lipopeptide-2 (MALP-2) induces the expression of heme oxygenase-1 (HO-1) in THP-1 cells and its possible mechanism. Methods: Human monocyte cells THP-1 were cultured in vitro and then were incubated with various concentrations (0, 0.01, 0.1, 1. 0 or 5.0 ng/ml) of MALP-2 for 16 h, or were stimulated by 5. 0 ng/ml MALP-2 for different length of time (0 h, 4 h, 8 h, 12 h, 16 h or 24 h). The expression of HO-1 at mRNA and protein levels were detected by real-time PCR analysis and Western blot ...