期刊论文

Wu, Y.-M.

中文

中华微生物学和免疫学杂志

0254-5101

2013

33

12

933-937

10.3760/cma.j.issn.0254-5101.2013.12.009

31270218:国家自然科学基金 81202323:国家自然科学基金 2010-212:湖南省高等学校科技创新团队支持计划

本校为第一且通讯机构

目的：研究鹦鹉热嗜衣原体（ Chlamydophila psittaci，Cps） CPSIT＿0271蛋白的定位和表达时相，及其重组蛋白对人单核细胞（ THP-1）产生促炎症因子的诱生作用。方法原核表达、纯化GST-CPSIT＿0271重组蛋白，免疫BALB/c小鼠制备多克隆抗体，ELISA分析血清抗体滴度。间接免疫荧光法初步观察内源性CPSIT＿0271的定位，并通过Western blot 分析CPSIT＿0271在Cps感染HeLa细胞8 h、18 h、24 h、36 h和48 h后的表达情况。用不同浓度GST-CPSIT＿0271刺激THP-1细胞，ELISA检测IL-6、IL-1β和TNF-α水平。结果在Cps感染的HeLa细胞中，CPSIT＿0271分布于衣原体包涵体内。 CPSIT＿0271在Cps感染HeLa细胞后...

Objective: To study the intracellular localization and temporal expression of CPSIT_0271 in Chlamydia psittaci-infected cells; and to investigate the effects of recombinant GST-CPSIT_0271 protein on the expression of proinflammatory cytokines including IL-6, IL-1β and TNF-α by THP-1 cells. Methods: The gene encoding CPSIT_0271 of Chlamydia psittaci was expressed as fusion protein (GST-CPSIT_0271) in E. coli. The polyclonal antibody was prepared by immunizing BALB/c mice with the purified recombinant protein. Antibody titer was determined by E...