期刊论文

Wu, Y.-M.

中文

中华微生物学和免疫学杂志

0254-5101

2012

32

3

212-217

10.3760/cma.j.issn.0254-5101.2012.03.004

国家自然科学基金

本校为第一且通讯机构

目的建立沙眼衣原体(Chlamydia trachomatis,Ct)D型感染小鼠生殖道动物模型,为研究人类生殖道Ct感染的病理变化和致病机制提供实验基础.方法分离20个临床株,经阴道接种C3H/HeJ小鼠,得到清除时间明显延长的临床株(UT0603).应用该临床株建立Ct D型感染模型,免疫荧光检测阴道脱落上皮细胞中衣原体的含量；原位组织免疫荧光检测上生殖道的衣原体感染定植情况；分离生殖道组织,肉眼观察并进行病理学评分.结果Ct临床株感染小鼠下生殖道其排菌量及排菌周期明显延长,可见衣原体有上行感染和定植,并能引发类似人类生殖道感染的病理变化.结论成功建立了Ct D型生殖道感染动物模型.

Objective: To construct a mouse model for studying pathophysiology and mechanism of human Chlamydia trachomatis genital infection. Methods: Innate immunity-deficient C3H/HeJ female mice were infected intravaginally with human C. trachomatis serovar D urogenital isolates for screening the highest violent clinical strain. The clinical strain UT0603 as well as standard strain D/UW-3/CX were then used to reinfect naive mice, the lower genital tract shedding were monitored by swabbing every 3-7 day over the entire infection period by culture. Some mice were sacrificed at early infection stage to de...