期刊论文

Zhang, Y.

中文

中华微生物学和免疫学杂志

0254-5101

2011

31

2

140-145

10.3760/cma.j.issn.0254-5101.2011.02.010

湖南省自然科学基金课题（06JJ2093） 湖南省高校科技创新团队资助项目（湘教通[2010]212号）

本校为第一且通讯机构

目的 探讨壳聚糖包裹DNA疫苗黏膜免疫效果.方法 采用复凝聚法制备载幽门螺杆菌脂蛋白 Lpp20基因的壳聚糖(CS)纳米粒(NPs),并对CS/DNA纳米粒的特质进行研究;用载基因壳聚糖纳米粒黏膜免疫(滴鼻和口服)小鼠,检测免疫小鼠的细胞和体液免疫水平.结果 裸质粒pcDNA3.1(+)/Lpp20与CS/DNA NPs通过黏膜免疫均能诱导小鼠产生有效的免疫应答.CS/DNANPs滴鼻和口服免疫组诱导的抗体明显升高,与裸质粒组相比有明显差异(P＜0.05),同时CS/DNANPs滴鼻和口服免疫组小鼠脾淋巴细胞经特异性抗原刺激后,刺激指数及培养上清中IFN-γ和IL-4含量明显高于裸质粒组、壳聚糖组和PBS组,且滴鼻免疫组高于口服组.结论 壳聚糖纳...

Objective: To investigate the immune response of mucosal immunization of new chi-tosan(CS) nanoparticles coating DNA vaccine. Methods: The chitosan nanoparticles containing plasmid DNA encoding H. pylori lipoprotein Lpp20 gene were prepared using a complex coacervation method and then its speciality were analyzed. We then administered the naked plasmid DNA and chitosan-DNA nanoparticles to 6-week-old female BALB/c mice by intranasal or oral mucosal routes to observe the humoral and cellular immune responses. Results: Naked plasmid pcDNA3.1(+)/Lpp20 and chitosan-pcDNA3.1(+)/Lpp20 nanoparticles ...