期刊论文

Zhang, Y.

中文

中华微生物学和免疫学杂志

0254-5101

2009

29

5

454-459

10.3760/cma.j.issn.0254-5101.2009.05.017

湖南省自然科学基金资助项目（06JJ2093） 湖南省教育厅青年基金资助项目（08B067）

本校为第一且通讯机构

目的 研究幽门螺杆菌(Helicobacterpylori)空泡毒素(VacA)单一毒力决定簇对THP-1巨噬细胞分泌和凋亡的影响,以及核因子KB(nuclear factor KB,NF-KB)在调节VacA诱导的THP-1巨噬细胞功能中的作用.方法 将pDsRed-Monomer-Cl/vacA转染THP-1巨噬细胞,ELISA法检测巨噬细胞培养上清IL-1β、TNF-α含量;Griess试剂分析培养上清的一氧化氮(NO)水平;荧光探针DCFH-DA检测培养上清的活性氧(ROS);流式细胞仪检测细胞的凋亡率;凝胶阻滞试验(electro-phoretic mobility gel shift assay,EMSA)检测NF-kB的核转位.结果 重组质粒转染后6 h,重组质粒组培养上清中TNF-α、IL-1β含量明显高于阴性对照组(P

Objective: To study the effect of VacA on the secretion of THP-1 macrophages as an individual virulence determinant, and the effect of NF-κB on the secretion of THP-1 macrophages. Methods: The recombinant plasmid pDsRed-Monomer-C1/vacA was transfected into macrophages. The cytokine content of TNF-α or IL-1β in the culture medium was tested quantitatively with ELISA kit, respectively. The content of NO or ROS in the culture medium was tested with Griess reagent or DCFH-DA fluorescent probe. The apoptosis rate of macrophages was tested by flow...