期刊论文

Wu, Y.-M.

中文

中华微生物学和免疫学杂志

0254-5101

2004

24

10

807-811

湖南省自然科学基金

本校为第一且通讯机构

目的了解穿透支原体潜在的致病性和穿透支原体脂质相关膜蛋白诱导小鼠巨噬细胞表达诱导性一氧化氮合酶(iNOS)的分子机制. 方法用从穿透支原体提取的脂质相关膜蛋白刺激小鼠巨噬细胞,以RT-PCR和Western blot等方法分析诱导性一氧化氮合酶的表达和NO的产生.用间接免疫荧光和Western blot检测经穿透支原体脂质相关膜蛋白处理的小鼠巨噬细胞中NF-κB的激活和NF-κB抑制剂PDTC(吡咯啉烷二甲基硫脲)对iNOS的表达和NO产生的影响. 结果穿透支原体脂质相关膜蛋白以时间和剂量依赖方式刺激小鼠巨噬细胞产生NO,且能激活NF-κB诱导巨噬细胞表达iNOS的mRNA和蛋白,其mRNA和蛋白的表达水平能被PDTC所抑制. 结论...

Objective: To investigate the potential pathogenicity of Mycoplasma penetrans (M. penetrans) and the molecular mechanisms that is responsible for the induction of inducible nitric oxide synthase(iNOS) gene expression in mouse macrophages stimulated by lipid-associated membrane proteins (LAMPs) prepared from M. penetrans. Methods: Mouse macrophages were stimulated by M. penetrans LAMPs to analyze the production of nitric oxide(NO), the expression of iNOS was detected by RT-PCR and Western blot. The activity of nuclear factor κB(NF-κB) and the ...