期刊论文

中文

中国癌症杂志

1007-3639

2011

21

2

99-102

10.3969/j.issn.1007-3969.2011.02.004

国家自然科学基金资助项目（No：81071966） 湖南省高校创新平台开放基金项目（No 09K074） 湖南省高校重点实验室（肿瘤细胞与分子病理学）资助.

本校为第一机构

背景与目的:维甲酸相关孤核受体α(retinoid acid receptor related orphan receptorα,RORα)可能参与肿瘤的调控.本实验室前期研究发现,二烯丙基二硫(diallyl disulfide,DADS)可抑制人胶质瘤U251细胞增殖,其抑制增殖作用可能与诱导RORα蛋白表达上调有关.为了明确RORα在DADS抑制人胶质瘤细胞增殖中的作用,本研究采用miRNA干扰技术抑制RORα表达,观察其对DADS抑制U251细胞增殖的影响.方法:首先将RORαmiRNA转染人胶质瘤U251细胞,运用Western blot检测转染前后RORα蛋白表达情况.实验分为未转染组,脂质体转染组和RORαmiRNA转染组及分别经30 mg/L DADS处理后的3组,共6组.MTT法检测RORα表达下调...

Background and purpose： RORα protein may play a role in regulating tumor growth. In a previous study, growth inhibition was seen in glioblastoma cell line U251 treated with diallyl disulfide （DADS）, where involvement of up-regulated ROR alpha protein is suspected. In this study, miRNA that targeted the RORα gene was transfected into U251 cells in order to supply theoretical evidence for the role of RORα as a new target of malignant glioma in the DADS-induced inhibition effect. Methods： RORα miRNA plasmid was transfected into human brain...