Objective: In order to find the best extract condition for the isolation of polysaccharide from spirulina. Methods: Single factors such as time, temperature, content of C2H5OH were studied for their effect on the process of polysaccharide's extraction. And then the extraction condition was optimized with orthogonal test. Results: The optimized extraction condition has got: 80℃, 3 hr, twice, and 75 % C2H5OH. Conclusions: Temperature has remarkable effect on the ratio of extraction....