期刊论文

Expression and significance of tight junction proteins in the kidney in a mouse model of renal ischemia-reperfusion injury

Li, Z.-H.

中文

中国当代儿科杂志

1008-8830

2018

20

12

1055-1062

10.7499/j.issn.1008-8830.2018.12.015

(2016SK4004):湖南省科技创新平台与人才计划项目 (2014SK2008):湖南省科技厅重点项目

本校为第一机构

目的 探讨紧密连接蛋白claudin-2、claudin-10和claudin-17在缺血再灌注肾损伤模型小鼠肾脏中的表达及意义.方法 将152只雄性C57BL/6小鼠随机分为对照组(n=8)、假手术组(n=72)及模型组(n=72).采用夹闭小鼠双侧肾蒂30 min的方法建立缺血再灌注肾损伤小鼠模型,假手术组及模型组依据再灌注后时间点(0、3、6、12、24、48、72 h及5 d、7 d)分为9个亚组,每组8只小鼠.分别采用RT-PCR法及免疫组化法分别检测各组小鼠肾组织紧密连接蛋白claudin-2、-10、-17 mRNA及其蛋白表达水平.结果 对照组及假手术组小鼠肾组织claudin-2、-10、-17 mRNA及其蛋白表达水平随再灌注时间点无明显变化(P>0.05).与对照组及假...

Objective To study the expression and significance of tight junction proteins (claudin-2, claudin-10, and claudin-17) in a mouse model of renal ischemia-reperfusion injury. Methods A total of 152 male C57BL/6 mice were randomly assigned to control group (n=8), sham-operation group (n=72), and model group (n=72). The renal pedicles at both sides were clamped for 30 minutes to establish a mouse model of renal ischemia-reperfusion injury. According to the time points of reperfusion (0, 3, 6, 12, 24, 48, and 72 hours and 5 and 7 days), the sham-ope...