期刊论文

Liu, Y.

中文

中国寄生虫学与寄生虫病杂志

1000-7423

2013

31

1

27-32

1教育部博士点基金博导类资助项目（No.20110162110029） 2国家自然科学基金（No.81101274） 3湖南省自然科学基金（No.10JJ3010） 4湖南省教育厅优秀青年项目（No.118106）

本校为第一机构

目的筛选噬菌体十二肽库中与日本血吸虫(Schistosoma japonicum)脱尾童虫表膜特异性结合而不与尾蚴表膜结合的短肽并鉴定。方法利用M13噬菌体十二肽库,经体外逆向差异筛选日本血吸虫尾蚴和脱尾活童虫,从第3轮回收的结合噬菌体中随机挑取15个克隆进行测序。获取目标噬菌体后,采用ELISA法、洗脱回收率实验(以M13KE为阴性对照)和免疫组织化学法检测日本血吸虫脱尾童虫、尾蚴与噬菌斑克隆的特异性结合。荧光显微镜观察人工合成的阳性噬菌体短肽与日本血吸虫脱尾童虫体外的特异性结合。构建目的片段pEGFP-C2质粒体外转染日本血吸虫脱尾童虫。结果经3轮逆向差异筛选后,噬菌体回收率从第1轮的3.50×l0~...

OBJECTIVE: To screen and analyze the peptides in 12 phage-display peptide library specifically binding to the schistosomulum, not cercaria, tegument of Schistosoma japonicum. METHODS: A 12 phage-display peptide library was screened with the S. japonicum schistosomula and cercariae as the target cells for biopanning by degrees, 15 positive clones were picked randomly and deduced by DNA sequencing. According the sequencing result, ELISA test, elution recovery test and immunohistochemical staining were performed to determine the specificity of the phages to the tegument. To further examine its bi...