期刊论文

中文

中国寄生虫学与寄生虫病杂志

1000-7423

2012

30

5

415-417

国家自然科学基金（No.8107389,81101274） 湖南省教育厅青年项目（No.11B106）

本校为第一机构

将日本血吸虫用双层琼脂预包埋后制作琼脂块,利用乙醇、异丁醇和正丁醇脱水,石蜡包埋后常规切片、脱蜡,即可进行各种染色步骤。该法可获得完整的纵向虫体连续切片,且容易对预定切面进行准确定位。

Schistosoma japonicum adults are pre-embedded in a double-layer agar and made the block, then dehydrated with alcohol, isobutyl alcohol and n-butyl alcohol. Various staining procedures can be conducted after conventional sectioning and dewaxing. Complete longitudinal serial sections of the pre-embedded worms can be obtained, and the desired se...